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Standard Operating Procedures Laboratory Specific – Biochemistry Shared Instrumentation Facility Centrifuges – All sizes and speeds _____ Department: Chemistry and Biochemistry Date when SOP was written: Sept 20, 2012 Date when SOP …
1.1 SOP: Standard Operating Procedure . 1.2 CRF: Case Report Form . 2. Purpose / Background 2.1. The purpose of this SOP is to provide guidance for the processing, testing and storage of all PERCH study 1 Centrifuge the plain blood tube at 3000 rpm for 10 min to separate blood cells 2 Transfer serum in 500 μL aliquots into Sarstedt tubes
Jul 18, 2019· Centrifuge rotor speed is often expressed as RCF in units of gravity (x g) for various procedures. However, many centrifuges display speed as revolutions per minute (RPM), necessitating conversion to ensure the correct experimental conditions. The following formula is used to convert RPM to RCF, where R is the rotor radius (cm) and S is the
Standard Operating Procedure Use and Maintenance of the Eppendorf Centrifuge . Document ID: HSL_EQ_020 . Version 1.0 Page 7 of 10 . 8.5.3. Attach and tighten rotor lid. 8.6. Loading Swing-Bucket Rotor: Note: When using the A-2-MTPs, centrifuge without the upper shell of the wind shield in order to guarantee precise and quick temperature control
SOP.008 Operation of the Fixed Angle Centrifuge Updated June 16, 2020 - Page 1 of 2 Purpose The purpose of this SOP is to provide instruction in the proper use of the fixed angle centrifuge. Materials 1. 70% ethanol 2. Kimwipes Methods 1. Select the appropriate rotor for the run. Wipe down the rotor and centrifuge chaer with 70% ethanol.
Standard operating procedure of refrigerator centrifuge 1. Purpose: This instruction describes how to use and maintain a refrigerated centrifuge. 2. Operating range: This device is for centrifugation in a maximum volume of 50ml. 3. Responsibility: The laboratory expert is responsible for the correct operation of
SOP.008 Operation of the Fixed Angle Centrifuge Updated June 16, 2020 - Page 1 of 2 Purpose The purpose of this SOP is to provide instruction in the proper use of the fixed angle centrifuge. Materials 1. 70% ethanol 2. Kimwipes Methods 1. Select the appropriate rotor for the run. Wipe down the rotor and centrifuge chaer with 70% ethanol.
This standard operating procedures (or SOP) describes methods for processing specimens of cerebrospinal fluid (CSF), lymph nodes and tissues for testing using the Xpert MTB/RIF assay and for purposes of culturing Mycobacterium tuberculosis culture on solid media or liquid media. Centrifuge at 3000 g for 15 minutes. Carefully pour off the
Centrifuge maintenance is critical to keep centrifuge users safe. The centrifuges that require regular (monthly) maintenance are Sigma 2-16 (9.129), Eppendorf 5810 (9.136), Sigma 4-16KS (9.134), and Thermo IEC CL10 (8.130a). Other smaller centrifuges do not require this. All centrifuges receive annual or biannual professional maintenance.
CRF-LAB-SOP-1 v4.0 5.1.8 Stay with the centrifuge until it gets up to its set speed. If the blood tubes are not properly balanced or there is a problem with a bucket or the rotor, the centrifuge will make an unusual noise and shake. If this happens, stop the centrifuge, open the lid and assess what caused the ialance, correct
ASE T Centrifuges Faculty of Technology and Trades Rooms: WA129 and WA130 Date Created: 04-June-14 Date Modified: May 2018 1.0 Purpose Version #: 2.0 Supersedes: 1.0 Prepared by: Applied Science and Environmental Technology (ASET) representative This standard operating procedure (SOP) provides general instructions for the safe operation,
The goal of this SOP is to train personnel in the safe operation of high speed centrifuges, both fixed rotor and swinging bucket rotor types. This SOP does not apply to microcentrifuge units. SCOPE. SOP applies to everyone who is working with centrifuges. MATERIALS NEEDED. Centrifuge tubes and caps. Millipore water for balancing. Two pan
2.1 The purpose of this Standard Operating Procedure (SOP) is to describe the procedures for the safe operation and routine maintenance of the Eppendorf 5702R and Labofuge 400R model centrifuges which are loed within the Experimental Medicine Facility (EMF) and Clinical Trials Facility (CTF). 3.0 Scope
2. Unplug the centrifuge and allow the rotor to completely stop; 3. Allow 20-30 minutes for any aerosols that were generated to settle before opening the centrifuge; 4. Put a visible sign to inform others about the spill in the centrifuge; 5. Be sure to choose the right disinfectant that will not damage the centrifuge and parts.
This SOP describes the procedure for operation and cleaning of Centrifuge. Procedure: Operation Connect the three pin plug with the main supply. To ensure that the rotor has been correctly loaded with its components of tube and buckets, the centrifuge can be started by closing its lid. Now move the speed control knob clock wise from “0” position.
In this video SOP, ABPDU staff describe how to set up and operate the Disc Stack Centrifuge system for use in processing material generated from microbial fe
STANDARD OPERATING PROCEDURE A BIOFIZZ PRODUCTON Centrifuge 1 Module Hours: 3.0 Effective Date: 01/17/2022 PRQs Lab Safety Pipetting Revision # 1.0 M. Stowell Checked: Z. Hazlett-Klein, M. Guzie - 4 - 6.3.3. For a continuous run (hold), turn the time dial clockwise to the limit stop. Two minus signs (–) will be illuminated in the time display.
2 Set the centrifuge timer at a setting frequently used in procedures, and start the NIST stopwatch simultaneously. 3 Stop the NIST stopwatch at the same time as the centrifuge timer ends. 4 Record both times of the stopwatch and the timer setting, as accurately as possible, on the Centrifuge Calibration Verifiion Record Sheet.
After the centrifuge has started the lid key will become disabled and the red light will be unlit. 27. Once the centrifugal run is complete the investigator or delegated person must open the lid of the centrifuge by pressing lid key on the on the front panel of the centrifuge, when there is a yellow light this indies that the lid
{Open the lid of the centrifuge and check for condensation. If the centrifuge is wet and/or dirty, clen it out including the spindle. {Check that the main large seal on the top of the centrifuge is clear of obstructions. {Using the OVERTEMP knob, set the red bar of the temperature meter well above the aient temperature. {Turn the POWER switch ON.
9. Centrifuge at 13.000 x g for 5 minutes. 10. Pour off the supernatant. Add 700 µl of Ethanol 70% and invert the tubes to wash DNA pellets. 11. Centrifuge at 13.000 x g for 5 minutes. Carefully pour off ethanol. The pellet may be loose so pour slowly and watch the pellets. 12.
Jul 27, 2008· 5.1.3 Remove the centrifuge head once in a month, apply grease at threading and fix it properly. 5.2 PROCEDURE FOR OPERATION. 5.2.1 Keep the instrument on stable platform. 5.2.2 Before operating the instrument, the speed knob and timer must be in ‘OFF’ position. 5.2.3 Put the main switch and the instrument switch to ‘ON’ position.
Apr 15, 2021· Fill and open centrifuge tubes, rotors, and accessories in a biological safety cabinet (BSC). Use sealed tubes and safety buckets that seal with O-rings. Before each use inspect tubes, O-rings, and buckets for cracks, chips, erosions, bits of broken glass, etc. Do not use aluminum foil to cap centrifuge tubes—it may detach or rupture during
In this video SOP, ABPDU staff describe how to set up and operate the Disc Stack Centrifuge system for use in processing material generated from microbial fe
STANDARD OPERATING PROCEDURE A BIOFIZZ PRODUCTON Centrifuge 1 Module Hours: 3.0 Effective Date: 01/17/2022 PRQs Lab Safety Pipetting Revision # 1.0 M. Stowell Checked: Z. Hazlett-Klein, M. Guzie - 4 - 6.3.3. For a continuous run (hold), turn the time dial clockwise to the limit stop. Two minus signs (–) will be illuminated in the time display.
This SOP describes the optimal operation of a centrifuge through regular servicing and preventive maintenance. Definitions and abbreviations The relative centrifugal force (RCF) is determined according to the formula RCF = 1.12 x 10–6 x R x (rpm)2 where: RCF is in g R
Centrifuges are a very powerful tool used for many routine procedures in the biological laboratory. Centrifuges can spin at extremely high rotations per minute (rpms) and impart great energy onto samples, thus increasing the risk of aerosol generation. This equipment can also present a physical hazard due to mechanical stress or fatigue. When utilizing centrifuges […]
2. Unplug the centrifuge and allow the rotor to completely stop; 3. Allow 20-30 minutes for any aerosols that were generated to settle before opening the centrifuge; 4. Put a visible sign to inform others about the spill in the centrifuge; 5. Be sure to choose the right disinfectant that will not damage the centrifuge and parts.
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