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centrifuge operating procedure

SOP - OPERATION AND CALIBRATION PROCEDURE FOR CENTRIFUGE …

Jul 27, 2008· 5.1.3 Remove the centrifuge head once in a month, apply grease at threading and fix it properly. 5.2 PROCEDURE FOR OPERATION. 5.2.1 Keep the instrument on stable platform. 5.2.2 Before operating the instrument, the speed knob and timer must be in ‘OFF’ position. 5.2.3 Put the main switch and the instrument switch to ‘ON’ position.

Safe Work Practices Centrifuge Use

Standard Operating Procedure (SOP) A Standard Operating Procedure is a detailed step-by-step procedural document on the safe operation of the particular centrifuge used by your lab. It should contain precise, practical instructions on how to use the device and all the safety measures that should be followed including PPE requirements. It

CENTRIFUGE SAFETY - California State University, Fullerton

STANDARD OPERATING PROCEDURE GUIDANCE The following information may be integrated into a lab-specific standard operating procedure (SOP) for centrifuge use. 1. PLANNING FOR USE • Complete lab-specific training for the centrifuge. • Wear appropriate PPE: Including safety eyewear, gloves, lab coat, and appropriate street

Standard operating procedure of refrigerator centrifuge

Method of work: 1. Turn on the device and turn it on with the key on the side of the device. 2. Press the blue key on the right side of the device to open the lid of the device. 3. Based on the size of the Falcon, we place the appropriate rotor in the device. We place the samples in the machine so that they are facing each other.

Working with Centrifuges: Operation and Safety Measures

The operating controls and indior dials for speed and time are mounted on the cabinet. Most centrifuges have a brake system to bring the rotor to a standstill shortly after the run isfinished. Many centrifuges are also refrigerated to prevent delie biological samples from getting warm, since samples tend to heat up during the

Centrifuge Safety SOP-CS-01

Version 1 (Deceer 1, 2017) - 1 – SOP-CS-01 Centrifuge Safety SOP-CS-01 . Standard Operating Procedure Centrifuge Safety SOP Nuer: SOP-CS-01 egory: Lab Equipment Supersedes: N/A Effective Date: Deceer 1, 2017 Pages 8 Subject: CENTRIFUGE SAFETY . Prepared By: Lisa McAvoy 16/Nov/17

Standard Operating Procedure - Centrifuge - Term Paper

The goal of this SOP is to train personnel in the safe operation of high speed centrifuges, both fixed rotor and swinging bucket rotor types. This SOP does not apply to microcentrifuge units. 2. SCOPE. SOP applies to everyone who is working with centrifuges. 3. MATERIALS NEEDED. 1. Centrifuge tubes and caps.

Centrifuge Safety – Stanford Environmental Health & Safety

Emergency procedures Mechanical Failure Turn off centrifuge immediately and unplug power cord. Do not use centrifuge again until inspected by qualified service technician. Hazardous Material Spill/Exposure Turn off centrifuge immediately. Keep centrifuge cover closed for at least 30 minutes to reduce aerosolization of hazardous material.

The Basics of Centrifuge Operation and Maintenance | Lab

Nov 03, 2020· Using the Centrifuge Download Article 1 Place the centrifuge on a firm, level surface. Because of the high speeds at which the centrifuge spins, it needs to be housed on a solid, flat surface. Find a level table or counter that is secure and can properly support the weight of the centrifuge.

Proper Use and Care of the Medical Laboratory Centrifuge

Compute Rotations per Minute (RPM) to Relative Centrifugal Force (RCF) and vice versa and illustrate when this procedure might be required. Explain the process and safety precautions that should be followed when operating a centrifuge. Give examples of ways to maximize the performance of centrifuges with scheduled maintenance. Customer Ratings

Operating Centrifuges

SOP-Centrifuges b. We have one large benchtop centrifuge: a Sorvall Legend i. There are two sets of buckets for the Legend: 1. 75006441 K: maximum speed 4600 rpm; maximum load 800 g 2. 75006449 Q: maximum speed 4600 rpm; maximum load 500 g 3. Inspect the rotor (or buckets) for signs of corrosion or cracking. If found, do not use the rotor

Instruction Manual for Table Top Centrifuge Z 383

• Under no circumstances open the lid of the centrifuge while the rotor is still running or rotating with a speed of > 2 m/s. Following rules must strictly be adhered to: • Do not operate the centrifuge in case it is not installed correctly. • Do not operate the centrifuge when dismounted (e.g. without metal cover).

Centrifuge Machine Calibration SOP - Pharmaceuticals

6.0 PROCEDURE: 6.1 Ensure that all the connections of instrument are proper. 6.2 Operate the instrument as per the operating instructions given in SOP. 6.3 Calibration of time: 6.3.1 Set the time for 5 minutes using set parameters. 6.3.2 Record the results by using digital stop watch.

Centrifuge and Rotor SOP - InsideEWU

Standard Operating Procedure Page 1 of 5 Origin Date: 5/8/2015 Revision 3 Revision Date: 2/2/2017 Introduction The purpose of this Standard Operating Procedure (SOP) is to provide guidance for the safe operation of centrifuges and rotors in the laboratory. Centrifuges are a commonly used piece of laboratory equipment. Centrifuges spin at high

Centrifuges: Types, Classifiions, Appliions, and

The centrifuge should also have an option for setting desired settings so that it can be left to function automatically without having to manually change configurations or need human supervision. Rotor Ialance. An ideal centrifuge should have sensors that detect any rotor ialance to avoid spillage and disruptions of separating procedures.

How to Use a Centrifuge Safely - University of California

Apr 15, 2021· Fill and open centrifuge tubes, rotors, and accessories in a biological safety cabinet (BSC). Use sealed tubes and safety buckets that seal with O-rings. Before each use inspect tubes, O-rings, and buckets for cracks, chips, erosions, bits of broken glass, etc. Do not use aluminum foil to cap centrifuge tubes—it may detach or rupture during

Standard Operating Procedure: Peripheral Blood …

6.7 Centrifuge that meets the following requirements: • Centrifuge must be capable of generating at least 1500-2000 x g (RCF) at the tube bottom. • Centrifuge with Swinging Bucket Rotor. • Tube buckets/adapters for 13 x 100mm and/or 16 x 125mm for selected CPT tube size and 16 - 24 x 50mL conical tubes (CSTFB separation).

Centrifuge: Introduction, Principle, Types, Handling

Biological centrifugation is a technique that uses centrifugal forces to separate and purify a mixture of biological particles in a liquid medium. It is a key method for isolating and analyzing the cells, sub-cellular fractions, supra molecule complexes, and isolated macro-molecules such as proteins and nucleic acids. Parts of Centrifuge

Centrifuge 5804/5804 R Centrifuge 5810/5810 R - Eppendorf

The Centrifuge 5804/5804 R/5810/5810 R is used for the separation of aqueous solutions and suspensions of different densities in approved sample tubes. The Centrifuge 5804/5804 R/5810/5810 R is exclusively intended for use indoors. All country-specific safety requirements for operating electrical equipment in the laboratory must be observed.

Standard Operating Procedure

In situations where risk group 2 agents will be centrifuged, centrifuge safety cups should be used. Tubes that are appropriate for the speeds and rotors of that particular centrifuge should be utilized. 5 PROCEDURE 5.1 General Safety Measures • Centrifuges are instruments with strong potential for harming users due to the high speed at

Centrifuge Safety – Laboratory Safety

Train each operator on proper operating procedures, review the user manual. Use only rotors compatible with the centrifuge. Check the expiration date for ultracentrifuge rotors. Check tubes, bottles, and rotors for cracks and deformities before each use. The operator should not leave the centrifuge until full operating speed is attained and

Centrifuge Safety SOP-CS-01

Version 1 (Deceer 1, 2017) - 1 – SOP-CS-01 Centrifuge Safety SOP-CS-01 . Standard Operating Procedure Centrifuge Safety SOP Nuer: SOP-CS-01 egory: Lab Equipment Supersedes: N/A Effective Date: Deceer 1, 2017 Pages 8 Subject: CENTRIFUGE SAFETY . Prepared By: Lisa McAvoy 16/Nov/17

Instruction Manual for Table Top Centrifuge Z 383

• Under no circumstances open the lid of the centrifuge while the rotor is still running or rotating with a speed of > 2 m/s. Following rules must strictly be adhered to: • Do not operate the centrifuge in case it is not installed correctly. • Do not operate the centrifuge when dismounted (e.g. without metal cover).

6 Safety Tips for Operating a Centrifuge | Lab Manager

There are a few important guidelines for operating a centrifuge, even a small one. Following them can prevent damage to the centrifuge and possible serious injury to you and others. 1. The work surface must be level and firm. Do not use the centrifuge on an uneven or slanted work surface. 2. Balance the tubes in the rotor!

Standard Operating Procedures - University of California

Standard Operating Procedures Laboratory Specific – Biochemistry Shared Instrumentation Facility Centrifuges – All sizes and speeds _____ Department: Chemistry and Biochemistry Date when SOP was written: Sept 20, 2012 Date when SOP was approved by the lab supervisor: _____ Supervisor Name and Signature: Margot Quinlan _____

Centrifuge Machine Calibration SOP - Pharmaceuticals

6.0 PROCEDURE: 6.1 Ensure that all the connections of instrument are proper. 6.2 Operate the instrument as per the operating instructions given in SOP. 6.3 Calibration of time: 6.3.1 Set the time for 5 minutes using set parameters. 6.3.2 Record the results by using digital stop watch.

Standard operating procedure of refrigerator centrifuge

Standard operating procedure of refrigerator centrifuge 1. Purpose: This instruction describes how to use and maintain a refrigerated centrifuge. 2. Operating range: This device is for centrifugation in a maximum volume of 50ml. 3. Responsibility: The laboratory expert is responsible for the correct operation of

How to Use a Centrifuge Safely - University of California

Apr 15, 2021· Follow these guidelines to use a centrifuge safely and keep it in good repair. Serious hazards associated with centrifuging include mechanical failure and the creation of aerosols. Minimize risk of mechanical failure and aerosol hazards by following the guidelines below. Expand All Requirement for researchers Mechanical failure Aerosols

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