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    BHO extractor closed loop

    Sells 1 lb, 10 lb, 20 lb, 50 lb and other closed-loop extractors, which are made of stainless steel, are inexpensive and have high extraction efficiency.

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    SPD2L short path distillation

    Sells a complete set of SPD-2L mini short-path distillation equipment, which is widely used in pharmaceutical, laboratory fractionation and purification, food and beverage, essential oil and other industries.

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    RE5299 rotary evaporator

    Our company's rotary evaporator RE-5299 rotary evaporator uses high borosilicate glass, with sufficient storage, cheap and good quality.

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    RE201D 301 501 rotary evaporator

    RE-201D RE-301 RE-501 rotary evaporator is a small rotary evaporator (mini rotary evaporator), beautiful appearance, small and light, top-rotating sealed, good vacuum.

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    S80L 100L glass jacketed reactor

    Is specializes in manufacturing and selling laboratory large-capacity glass reactors. Double-layer glass reactors can be customized and processed according to customer needs. The design is unique, safe and convenient.

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    S10L 20L 30L 50L glass jacketed reactor

    Is specializes in manufacturing and selling S-10L S-20L S-30L S-50L laboratory glass reactors, which can perform various solvent synthesis reactions under constant temperature conditions.

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    FD03H freeze dryer

    Home lab freeze dryer is a mini vacuum freeze dryer. We are a freeze dryer manufacturer in China. We sell freeze dryers, which are very suitable for home use.

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    DZF6500 vacuum drying oven

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    KDM100 molecular distillation

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centrifuge at 400 x g

Lipid Droplets Fluorescence Assay Kit

9. Centrifuge the plate at 400 x g for five minutes. 10. Aspirate the supernatant. 11. Add 0.1 ml of Assay Buffer to each well. 12. Centrifuge the plate at 400 x g for five minutes. 13. Aspirate the supernatant. 14. Add 0.05 ml of the Nile Red Staining Solution to each well except background control wells. Incubate cells at room temperature for

Product Data Sheet

10. Centrifuge tubes at 300-400 x g at room temperature for five minutes, and discard the supernatant. 11. Add 2 mL of cell staining buffer. 12. Centrifuge tubes at 300-400 x g at room temperature for five minutes, and discard the supernatant. 13. Resuspend in 0.5 mL cell staining buffer then acquire the tubes on a flow cytometer. Caution: The

Rutgers New Jersey Medical School

Sep 15, 2020· Centrifuge tube at 400 x rcf for 5 minutes; Resuspend pellet with 1 ml R-10 media and count cells using Trypan Blue; Note: save some cells for flow cytometry for the determination of the transduction efficiency. Transfer the resuspended cells to a G-REX well containing R-10 media supplemented with IL-2 (200 U/ml) and IL-15 (5 ng/ml)

Centrifuges / Lab Centrifuges - MRC- Lab

If the RPM and rotor radius is known, a monogram chart can be used to determine the RCF. RCF (or g) can be calculated by using the following formula (Rickwood, 1984, p. 288): RCF=11.18×r× (N/1000)². r = the radius in centimeters from the center of the rotor to the sample during centrifugation. N = the rotor speed in RPM.

Faster Isolation of PBMC Using Ficoll-Paque - Eppendorf

7. Centrifuge the sample in the desired swing-out rotor at 400 x g and 20 °C for 30 min, selecting acceleration/ deceleration rates of 9/0 or 9/3*, respectively, at the setting “at set rpm”. TIP: Typically, the rotor brakes must be inactivated en-tirely in …

Flow Cytometry (FACS) Protocols - Tabriz University of

4. Centrifuge at 400 x g for 35 minutes at room temperature. During this step, the granulocytes, platelets and RBCs pellet to the bottom of the tube and the PBMC float over the Ficoll-Paque PLUS or Histopaque-1077. 5. Carefully aspirate the PBMC from the Ficoll-plasma interface. 6. Wash the PBMC with PBS at 300 x g at 4°C twice.

General Protocol & Storage - .NET Framework

a) Centrifuge 300 µL of fixed yeast cells in 70% ethanol at 400 x g. b) Resuspend yeast in 100 μL of Hybridization Buffer containing probe. Mix well by pipetting up and down. c) Incubate in the dark at 30 °C overnight. d) Add 100 µl of Wash Buffer A (see recipe above) to the hybridized yeast cells. Centrifuge at 400 x g for 5 minutes. Aspirate

RCF & RPM Calculator for Centrifuges | Hettich N America

RCF/RPM Calculator. Use the RCF or RPM calculator below to determine the RCF or RPM value for your centrifugation cycle. If you need help determining the radius of your centrifuge configuration, simply fill out the form below or give us a call at 1-866-370-4388. I need assistance.

Centrifuge: accuSpin 400 | Geophysical Laboratory

Centrifuge: accuSpin 400 Contact: Robert Hazen Loion: R-121 This centrifuge is built with a steel chassis, comes equipped with automated rotor ialance detection. It has: a temperature range of aient to 40°C; a programmable run time of 0 to 9 hours of continuous operation; a maximum capacity of 400 ml; top speed of 13,000 RPM (16,060 x g); power 115V (60Hz).

Cell Preparation for Flow Cytometry - Thermo Fisher Scientific

4. Centrifuge cells at 300-400 x g for 4-5 minutes at 2-8°C. Discard the supernatant. 5. Resuspend the cell pellet in PBS. 6. Centrifuge cells as in Step 4. 7. Repeat Steps 5 and 6. 8. …

Enrichment of PBMCs with monocytes - METHODS

Centrifuge 30 min at 400 x g without brakes. Collect the PBMC fraction and transfer it into a 50 ml tube. Fill the tube with PBS, centrifuge 10 min at 300 x g. Resuspend good in 50 ml PBS, centrifuge 10 min at 200 x g. Resuspend good in 50 ml PBS, centrifuge 10 min at 300 x g. Prepare continuous Percoll gradient .

Assay Kit Mediated Cytotoxicity 7-AAD/CFSE Cell- - Abcam

Centrifuge the cells at 400 x g for five minutes to pellet the cells and wash once with 1 ml of Assay Buffer. 3. Resuspend cell pellet in CFSE Staining Solution at a concentration of 106 cells/ml. Control target cells (target cells without CFSE or 7-AAD staining or target cells with 7-AAD staining only) should be resuspended in 0.1%

G-Force/RPM Centrifuge Conversion Calculator

RCF = Relative Centrifuge Force, in "g" r = Rotor radius, in "mm" rpm = Rotation speed, rotations per minute, in "rpm" For Enjoyable Protein Research NovoPro +86-21-61842887 86-213-536-6391 [email protected] Room 229, Building 26, No. 1333 Xinlong Road, Minhang District, Shanghai, China, Zip-code: 201101

What is the difference between the G and the RPM in

Jul 01, 2011· The x g is for the force (nuer of times the gravitational force) exerted on the centrifuge tube and its contents during centrifugation and is the basis of separation of …

PRF Centrifuge - ariston dental better answers

It offers a wide range of settings satisfying all preferences and protocols of growth factors procurement (speed up to 5.000 rpm, RCF up to 4.400 x g, etc.). It offers pre-programmed settings for acquiring the most known platelet concentrates and growth factors at the push of a button (A-PRF™ Plus, A-PRF liquid, i-PRF, i-PRF Plus, etc.).

Benchtop Centrifuges | Fisher Scientific

Fisherbrand™ Mini-Centrifuge 100-240V, 50/60Hz Universal Plug, Grey PROMO. Tool-free rotors and quick-spin options make the Fisherbrand Mini-Centrifuge fast and easy to use. The locking lid is functionally designed to fit in the palm of the hand for easy repeat opening and quick tube extraction. Pricing & Availability.

How to convert centrifuge speed values from xg to rpm …

The relationship between revolutions per minute (RPM) and relative centrifugal force (xg) is: g = (1.118 × 10-5) R S2 where g is the relative centrifugal force, R is the radius of the rotor in

Cytogenetic protocol: mitotic chromosome from cell cultures

Transfer cell suspension to a conical centrifuge tube and centrifuge for 5 minutes in a clinical bench-top centrifuge at 400 x g. Remove supernatant and gently add 2-3 ml of warm (37°C) …

RPM vs RCF Calculator - Thermo Fisher

RPM vs RCF Calculator. Perform custom calculations with our RPM and RCF calculator and find the g-force of your Thermo Scientific Fiberlite centrifuge rotor at any rpm.

Generation of Prostate Cancer Patient Derived Xenograft

Centrifuge at 400 x g for 30 min at RT (25 °C). Set deceleration to lowest setting to prevent mixing of solutions after separation. Acceleration may be set to any speed. After centrifugation, identify the thin white-grey stripe of PBMCs and CTCs sandwiched layer between plasma on top and separation solution at the bottom of the tube.

7-AAD/CFSE Cell-Mediated Cytotoxicity Assay Kit

9. Centrifuge at 400 x g for five minutes and aspirate the supernatant. 10. Resuspend the cells in 200-500 μl of Assay Buffer. 11. The cells are now ready for analysis with a flow cytometer and …

Assay Kit Mediated Cytotoxicity 7-AAD/CFSE Cell- - Abcam

Centrifuge the cells at 400 x g for five minutes to pellet the cells and wash once with 1 ml of Assay Buffer. 3. Resuspend cell pellet in CFSE Staining Solution at a concentration of 106 cells/ml. Control target cells (target cells without CFSE or 7-AAD staining or target cells with 7-AAD staining only) should be resuspended in 0.1%

Stimulation of Cytokine Production in Immune Cells

Centrifuge at 400 x g for 20 minutes at room temperature with the brake OFF. Harvest PBMC loed at the interface of the PBS and Ficoll-Paque ® medium layers into a fresh tube. Fill the tube with PBS to wash the cells. Centrifuge the cells at 300–400 x g for 4–5 minutes at 2–8°C. Discard supernatant.

Swing Bucket Centrifuge at Thomas Scientific

Includes Multifuge X1R Refrigerated Centrifuge, TX-400 Swinging Bucket Rotor, Thermo Scientific Nunc …7,000 x g with cushion adapter High-density polyethylene plug-seal screw cap Volume 200 mL Molded volume graduations Sterile Compact design fits table top and floor standing centrifuges with 250 or 500 mL swinging bucket rotors

Store at -20ºC, 4ºC & RT 7-AAD/CFSE Cell-Mediated

centrifuge at 400 x g for five minutes. (For staining in tubes, scale volumes up 5-fold). 6. Aspirate the supernatant. NOTE: If additional surface markers are to be assayed, staining can be inserted at this point in the protocol. 7. Resuspend the cells in 200 μl of 7-AAD Viability Dye (prepared as described in Solu-tions and Reagents) and mix

XF TM OMPLETE Exosome and cfDNA

Centrifuge the spin column together with the Eppendorf tube for 1 minute at 400 x g followed by 2 minutes at 5,800 x g. 10. To increase recovery yield, transfer the eluted buffer back to the spin column and let stand at room temperature for 2 minutes. Centrifuge for 1 minute at 400 x g, followed by 2 minutes at 5,800 x g. 11.

Centrifuge Spins Samples Up To 400g | Hackaday

Oct 09, 2015· G-force= 1.12 x R x (RPM/1000)² . R=130 mm RPM=1667 (half-rotation of the centrifuge takes 18 ms) The nuers all add up to 404g, what is the problem ?

G-Force Calculator - Drucker Diagnostics

How to Calculate G-Force: The Force exerted on a sample in a centrifuge is a function of the rotation speed of the centrifuge (RPM) and the radius of the rotor. If you know the RPM, the equations to calculate G-Force are: In Centimeters: RCF or G-Force = 0.00001118 x Rotor Radius x (RPM)². OR.

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